Differential Effects of Cytochalasin B on Cytokinesis in Parthenogenetically Activated Mouse Oocytes.
نویسندگان
چکیده
منابع مشابه
P-229: Chromosomal Analysis of Parthenogenetic Mouse Embryos Generated from In Vitro Activated Oocytes by Hydrostatic Pressure and Ethanol and Cytochalasin B
Background: Studies of preimplantation stage embryos by classic cytogenetic techniques have limitations, starting with the need for good metaphase stage when only one third of all analyzed embryos may show good quality metaphases. The incidence of chromosome anomalies in embryos produced by in vitro procedures is generally higher than that of embryos formed in vivo. Pressure specifically affect...
متن کاملEffect of genetic background and activating stimulus on the timing of meiotic cell cycle progression in parthenogenetically activated mouse oocytes.
With the aim of investigating the effects of oocyte genotype and activating stimulus on the timing of nuclear events after activation, oocytes collected from hybrid B6D2F1, inbred C57BL/6 and outbred CF-1 and immunodeficient nude (NU/+) females were activated using ethanol or strontium and fixed at various time-points. Meiotic status, spindle rotation and second polar body (PB2) extrusion were ...
متن کاملP-75: The Effect of Hydrostatic Pressure in The Presence of Different Concentrations of Extracellular Calcium, Ethanol and Cytochalasin B on Parthenogenetic Activation of Mouse oocytes
Background: The parthenogenetic of oocytes is important in cloning research, as artificial activation of oocytes is an essential component of nucleus transfer protocols. Hydrostatic pressure can act as a mechanical stimulator that rearranges egg contents. Ethanol promotes a single intracellular Ca2+ increase of greater and longer amplitude than the initial increase observed at fertilization. In...
متن کاملCytochalasin-b: Time-lapse Cinematographic Studies on Its Effects on Cytokinesis
L cells exposed to cytochalasin-B (cyto-B) show the normal development of deep cleavage furrows in both bipolar and multipolar cell divisions Due to the drug-induced inhibition of cellular motility, the resulting daughter cells do not move away from each other but reunite to form multinucleate cells. In mitotic cells from cultures exposed to cyto-B for long periods of time, vigorous blebbing an...
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ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 2002
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.48.31